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Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

DOI:
10.3791/55284-v

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08:52 min

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February 18, 2017

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, , , , ,
1Department of Medicine II,Saarland University Medical Center, 2Department of Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM),University of Saarland

Capitoli

  • 00:05Titolo
  • 00:58Liver Single Cell Suspension Preparation
  • 02:40Flow Cytometric Cell Count Determination and Cell Surface Staining
  • 04:30Magnetic Microbead-based Progenitor Cell Enrichment
  • 07:01Results: Representative Liver Progenitor Subset Analyses
  • 08:22Conclusion

Summary

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Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.

Tags

Liver Progenitor CellsLiver Digestion ProtocolFlow CytometrySurface Marker CombinationCell IsolationCell BiologyHematopoietic CellsEndothelial CellsMouse LiverCell SuspensionCell CountingPropidium Iodide

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