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Neuroscience

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JoVE Journal Neuroscience
Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light
 

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Article DOI: 10.3791/55176-v • 11:36 min • February 10th, 2017
February 10th, 2017 •
1,2,5, 3, 4, 1,2, *4, *5
1Department of Neuroscience of Disease, Center for Transdisciplinary Research, Niigata University, 2Brain Research Institute, Niigata University, 3Image and Data Analysis Facility, German Center for Neurodegenerative Diseases (DZNE), 4Graduate School of Life Science and Technology, Tokyo Institute of Technology (Titech), 5Dendrite Differentiation, German Center for Neurodegenerative Diseases (DZNE)
* These authors contributed equally

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Here we show how to quantify the number and spatial distribution of synaptic active zones in Drosophila melanogaster photoreceptors, highlighted with a genetically encoded molecular marker, and their modulation after prolonged exposure to light.

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Synaptic Modulation Drosophila Melanogaster Photoreceptors Prolonged Light Exposure Experimental Procedure Synaptic Dynamics Activation Conditions Synaptic Plasticity Molecular Composition Semi-automated Analysis Synapse Number Synapse Distribution Molecular Enrichment Fly Collection Acrylic Rack Light Exposure Constant Darkness Light-dark Cycle Constant Light Brain Dissection Staining Techniques Mounting Medium Microscope Slide
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