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Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy
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JoVE Journal Neuroscienze
Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

DOI:
10.3791/63308-v

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11:12 min

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December 01, 2021

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, , , ,
1Laboratory of Biophysics of Synaptic Processes,Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 2Department of Radiophotonics and Microwave Technologies,Kazan National Research Technical University named after A.N. Tupolev, 3Department of Normal Physiology,Kazan State Medical University

Capitoli

  • 00:05Introduction
  • 01:12Dye Loading Procedure
  • 03:28Video Capture with Confocal Microscopy
  • 06:14Video Processing
  • 07:56Video Analysis
  • 09:55Results: Mouse Nerve Terminals Loaded with the Ca2+ Indicator and Representative Trace of Calcium Signal
  • 10:18Conclusion

Summary

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The protocol describes the method of loading a fluorescent calcium dye through the cut nerve into mouse motor nerve terminals. In addition, a unique method for recording fast calcium transients in the peripheral nerve endings using confocal microscopy is presented.

Tags

Calcium TransientsNeuromuscular JunctionConfocal MicroscopyPresynaptic CalciumSynaptic TransmissionCalcium SignalingNeurodegenerative DiseasesLoading TechniqueSpatial-temporal ResolutionConfocal MicroscopeNerve TrunkNerve StumpDye Loading Solution

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