Rivista
/
Neuroscienze
/
In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons

In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons

DOI:
10.3791/2151-v

•

05:58 min

•

October 15, 2010

•

,
1Department of Biological Sciences,SUNY-University at Buffalo

Capitoli

  • 00:00Titolo
  • 00:49Introduction
  • 01:01Preparation for Dissection
  • 01:51Collection of Larvae
  • 02:16Live Dissection of Larvae
  • 04:18Live Imaging of Dissected Larvae
  • 05:00Representative Images
  • 05:37Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol discusses the live dissection of Drosophila larvae for the purpose of imaging the movement of GFP tagged axonal vesicles on microtubule tracks.

Tags

In Vivo VisualizationSynaptic VesiclesDrosophila Larval Segmental AxonsAxonal TransportNeurological DiseasesDissection ProtocolLive ImagingUAS-GAL4 MethodGFP-tagged APPSynaptotagmin VesiclesNeuronal GAL4 DriversFluorescently Tagged MarkersMitochrondriaMitoTrackerLysosomesLysoTrackerGFP-vesicle MovementParticle Movement

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Functional Analysis of the Larval Feeding Circuit in Drosophila

Laser Nanosurgery of Cerebellar Axons In Vivo

Live Imaging of Drosophila Larval Neuroblasts

Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

In Vivo Optical Calcium Imaging of Learning-Induced Synaptic Plasticity in Drosophila melanogaster

Read Article