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Neuroscience

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JoVE Journal Neuroscience
Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo
 

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Article DOI: 10.3791/54142-v • 11:42 min • June 19th, 2016
June 19th, 2016 •
1,2, 2, 1, 1,2
1National Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, 2Department of Biomedical Science, The University of Sheffield

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Sharp microelectrodes enable accurate electrophysiological characterization of photoreceptor and visual interneuron output in living Drosophila. Here we show how to use this method to record high-quality voltage responses of individual cells to controlled light stimulation. This method is ideal for studying neural information processing in insect compound eyes.

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Electrophysiological Method Intracellular Voltage Responses Drosophila Photoreceptors Interneurons Light Stimuli In Vivo Neuro-coding Field Visual Information Information Sampling Reference Microelectrode Glass Tubing Recording Electrode Fine Gradual Taper Light Microscope Electrode Inspection
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