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协议小鼠胚胎成纤维细胞直接转化为滋养层干细胞
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
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协议小鼠胚胎成纤维细胞直接转化为滋养层干细胞

7,508 Views

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08:57 min

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July 25, 2016

DOI:

10.3791/54277-v

DOI:
10.3791/54277-v

•

08:57 min
July 25, 2016

•

2 Views
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1Department of Developmental Pathology,Institute of Pathology, University of Bonn Medical School

Summary

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Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.

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Cite this Article

Kubaczka, C., Schorle, H. Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells. J. Vis. Exp. (113), e54277, doi:10.3791/54277 (2016).

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