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Isolement des cellules embryonnaires murines Hemogenic endothéliales
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1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center,Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine,Yale University School of Medicine, 3Department of Molecular and Cellular Biology,Baylor College of Medicine
Capitoli
- 00:05Titolo
- 01:12Mouse Embryo Yolk Sac (YS) or Aorta-gonad-mesonephros (AGM) Dissection
- 03:29Primary YS/AGM Tissue Digestion
- 04:37Nucleic Acid Dye- and Fluorescently Conjugated Antibody-labeling
- 06:19Results: Representative Flow Cytometric Identification of Hemogenic Endothelial Cells (EC), Hematopoetic Stem and Progenitor Cells (HSPC), and Non-hemogenic EC
- 07:43Conclusion
cellules souches et progénitrices hématopoïétiques (HSPC) proviennent de (hemogenic) des cellules endothéliales spécialisées au cours du développement, mais on sait peu sur le processus par lequel certaines cellules endothéliales spécifient pour devenir la formation du sang. Nous démontrons une méthode basée sur la cytométrie de flux permettant l'isolement simultané des cellules endothéliales hemogenic et HSPC de tissus embryonnaires murines.
