This content is Free Access.
Isolation of Murine Embryonic Hemogenic Endothelial Cells
11,178 Views
•
08:56 min
•
June 17, 2016
DOI:
1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center,Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine,Yale University School of Medicine, 3Department of Molecular and Cellular Biology,Baylor College of Medicine
Summary
Automatically generated
Hematopoietic stem and progenitor cells (HSPC) derive from specialized (hemogenic) endothelial cells during development, yet little is known about the process by which some endothelial cells specify to become blood forming. We demonstrate a flow-cytometry based method allowing simultaneous isolation of hemogenic endothelial cells and HSPC from murine embryonic tissues.
Read Article
Cite this Article
Fang, J. S., Gritz, E. C., Marcelo, K. L., Hirschi, K. K. Isolation of Murine Embryonic Hemogenic Endothelial Cells. J. Vis. Exp. (112), e54150, doi:10.3791/54150 (2016).
Copy