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Biologia dello sviluppo

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小鼠胚胎Hemogenic内皮细胞的分离

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Isolation of Murine Embryonic Hemogenic Endothelial Cells

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小鼠胚胎Hemogenic内皮细胞的分离

11,119 Views

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08:56 min

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June 17, 2016

DOI:

10.3791/54150-v

DOI:

10.3791/54150-v

•

08:56 min

June 17, 2016

•

7 Views

Jennifer S. Fang*¹, Emily C. Gritz*², Kathrina L. Marcelo³, Karen K. Hirschi¹

¹Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center,Yale University School of Medicine, ²Department of Pediatrics, Section of Neonatal-Perinatal Medicine,Yale University School of Medicine, ³Department of Molecular and Cellular Biology,Baylor College of Medicine

Summary

造血干细胞和祖细胞（HSPC）的发展过程中从专门（hemogenic）内皮细胞衍生，还知之甚少由一些内皮细胞指定成为血液形成的过程。我们展示了一个流式细胞仪为基础的方法，允许从鼠胚胎组织hemogenic内皮细胞和HSPC的同时分离。

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