This work presents a protocol for liquid handling and sample introduction to a microchannel for in situ time-of-flight secondary ion mass spectrometry analysis of protein biomolecules in an aqueous solution.
Yu, J., Zhou, Y., Hua, X., Zhu, Z., Yu, X. In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS. J. Vis. Exp. (108), e53708, doi:10.3791/53708 (2016).