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In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
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JoVE 杂志 化学
In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS

DOI:
10.3791/53708-v

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09:48 min

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February 15, 2016

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, , , ,
1Fundamental & Computational Sciences Directorate,Pacific Northwest National Laboratory, 2Wiley Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory

Chapters

  • 00:05Title
  • 01:03Cleaning and Sterilization of the SALVI Microchannel
  • 02:05Immobilization of the Protein Film in SALVI
  • 02:50Install SALVI into the ToF-SIMS Loadlock Chamber
  • 04:05ToF-SIMS Data Acquisition
  • 07:35Results: Dynamic Liquid Surface and Liquid-solid Interface Analysis
  • 09:02Conclusion

Summary

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This work presents a protocol for liquid handling and sample introduction to a microchannel for in situ time-of-flight secondary ion mass spectrometry analysis of protein biomolecules in an aqueous solution.

Tags

In Situ CharacterizationHydrated ProteinsWaterSALVIToF-SIMSBiomoleculesBiointerfaceSolid-liquid InterfaceCancer CellsDrug InteractionsBiofilmsFibronectinSilicon WaferSilicon Nitride Membrane

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