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蛋白表达的定量分析来研究宗族规范小鼠植入前胚胎
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JoVE Journal Biologia dello sviluppo
Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
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蛋白表达的定量分析来研究宗族规范小鼠植入前胚胎

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11:25 min

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February 22, 2016

DOI:

10.3791/53654-v

DOI:
10.3791/53654-v

•

11:25 min
February 22, 2016

•

2 Views
, , , ,
1Developmental Biology Program,Sloan Kettering Institute

Summary

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该协议提出对蛋白质表达的原位分析进行定量，单细胞研究在小鼠植入前胚胎沿袭规范的方法。必要的囊胚的汇编程序，整个安装蛋白的免疫检测，在一个共焦显微镜，和核分割和图像分析的样品的成像进行描述。

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Cite this Article

Saiz, N., Kang, M., Schrode, N., Lou, X., Hadjantonakis, A. Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos. J. Vis. Exp. (108), e53654, doi:10.3791/53654 (2016).

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