Rivista
/
Biologia dello sviluppo
/
דור של תאי גזע האינטגרציה נטול האדם המושרה pluripotent שימוש קרטינוציטים נגזר שיער
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
Please note that all translations are automatically generated. Click here for the English version.

דור של תאי גזע האינטגרציה נטול האדם המושרה pluripotent שימוש קרטינוציטים נגזר שיער

11,229 Views

•

08:36 min

•

August 20, 2015

DOI:

10.3791/53174-v

DOI:
10.3791/53174-v

•

08:36 min
August 20, 2015

•

12 Views
, ,
1Centre for Eye Research Australia & Department of Ophthalmology,University of Melbourne

Summary

Automatically generated

This manuscript provides a step-by-step procedure for the derivation and maintenance of human keratinocytes from plucked hair and subsequent generation of integration-free human induced pluripotent stem cells (hiPSCs) by episomal vectors.

Explore More Videos

Hair-derived KeratinocytesHuman Induced Pluripotent Stem CellsHiPSCsNon-invasiveReprogrammingEpisomal VectorsRegenerative MedicineDisease ModelingIn Vitro Drug ScreeningGene Therapy

Read Article

Cite this Article

Hung, S. S., Pébay, A., Wong, R. C. Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes. J. Vis. Exp. (102), e53174, doi:10.3791/53174 (2015).

Download .ris file

Copy

Share Video

.

Copy