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病毒介导的标签和内侧神经节隆起移植（MGE）细胞<em>在体内</em>研究
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies
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病毒介导的标签和内侧神经节隆起移植（MGE）细胞<em>在体内</em>研究

DOI:
10.3791/52740-v

•

09:21 min

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April 23, 2015

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, , , , ,
1Department of Psychiatry,University of California San Francisco, 2Department of Neurological Surgery,University of California San Francisco

Capitoli

  • 00:05Titolo
  • 01:37Lentiviral Labeling of MGE Cells
  • 03:52Transplantation of Transformed MGE Cells
  • 06:42Results: Viral Mediated Labeling and Transplantation of MGE Cells
  • 08:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

GABA能中间神经元皮质祖分散，开发和突触融入移植后的主机皮层。这些细胞可以移植在体内研究转基因GABA能前体的前容易地转导。这里，我们表明病毒标记技术到目标使用现有的Cre线和依赖于Cre的记者特定中间神经元亚群。

Tags

Viral-mediated LabelingMGE Cell TransplantationGABAergic Cortical InterneuronsCre-driver LinesIn Vivo StudiesMolecular ApproachesCell Autonomous ProgrammingLentiviral TransductionGenetic ManipulationCell-type Specific Resolution

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