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Developmental Biology

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JoVE Journal Developmental Biology
Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies
 

Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies

Article DOI: 10.3791/52740-v • 09:21 min • April 23rd, 2015
April 23rd, 2015 •
1, 1, 2, 2, 2, 1
1Department of Psychiatry, University of California San Francisco, 2Department of Neurological Surgery, University of California San Francisco

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GABAergic cortical interneuron progenitors disperse, develop and synaptically integrate into a host cortex after transplantation. These cells can be easily transduced before transplantation for in vivo studies of genetically modified GABAergic precursors. Here, we show viral labeling techniques to target specific interneuron subgroups using existing Cre lines and Cre-dependent reporters.

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Keywords: Viral-mediated Labeling MGE Cell Transplantation GABAergic Cortical Interneurons Cre-driver Lines In Vivo Studies Molecular Approaches Cell Autonomous Programming Lentiviral Transduction Genetic Manipulation Cell-type Specific Resolution
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