需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies
11,321 Views
•
09:21 min
•
April 23, 2015
DOI:
Summary
Automatically generated
GABAergic cortical interneuron progenitors disperse, develop and synaptically integrate into a host cortex after transplantation. These cells can be easily transduced before transplantation for in vivo studies of genetically modified GABAergic precursors. Here, we show viral labeling techniques to target specific interneuron subgroups using existing Cre lines and Cre-dependent reporters.
Read Article
Cite this Article
Vogt, D., Wu, P., Sorrells, S. F., Arnold, C., Alvarez-Buylla, A., Rubenstein, J. L. R. Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies. J. Vis. Exp. (98), e52740, doi:10.3791/52740 (2015).
Copy