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Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies

Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies

DOI:
10.3791/52740-v

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09:21 min

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April 23, 2015

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, , , , ,
1Department of Psychiatry,University of California San Francisco, 2Department of Neurological Surgery,University of California San Francisco

Kapitel

  • 00:05Titel
  • 01:37Lentiviral Labeling of MGE Cells
  • 03:52Transplantation of Transformed MGE Cells
  • 06:42Results: Viral Mediated Labeling and Transplantation of MGE Cells
  • 08:44Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

GABAergic cortical interneuron progenitors disperse, develop and synaptically integrate into a host cortex after transplantation. These cells can be easily transduced before transplantation for in vivo studies of genetically modified GABAergic precursors. Here, we show viral labeling techniques to target specific interneuron subgroups using existing Cre lines and Cre-dependent reporters.

Tags

Viral-mediated LabelingMGE Cell TransplantationGABAergic Cortical InterneuronsCre-driver LinesIn Vivo StudiesMolecular ApproachesCell Autonomous ProgrammingLentiviral TransductionGenetic ManipulationCell-type Specific Resolution

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