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免疫细胞健康和疾病肝脏使用CXCR6.Gfp记者小鼠长期活体多光子显微镜成像
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
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免疫细胞健康和疾病肝脏使用CXCR6.Gfp记者小鼠长期活体多光子显微镜成像

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11:44 min

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March 24, 2015

DOI:

10.3791/52607-v

DOI:
10.3791/52607-v

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11:44 min
March 24, 2015

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1Department of Medicine III,RWTH University-Hospital Aachen, 2IZKF Aachen Core Facility "Two-Photon Imaging",RWTH University-Hospital Aachen, 3Institute for Laboratory Animal Science & Experimental Surgery,RWTH Aachen University, 4Institute for Pharmacology,RWTH University-Hospital Aachen

Summary

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Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.

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Cite this Article

Heymann, F., Niemietz, P. M., Peusquens, J., Ergen, C., Kohlhepp, M., Mossanen, J. C., Schneider, C., Vogt, M., Tolba, R. H., Trautwein, C., Martin, C., Tacke, F. Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice. J. Vis. Exp. (97), e52607, doi:10.3791/52607 (2015).

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