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Neuroscience

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JoVE Journal Neuroscience
The Neuromuscular Junction
 

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

Article DOI: 10.3791/52220-v • 12:18 min • December 26th, 2014
December 26th, 2014 •
1, 2, 1, 3, 1, 1, 1
1Physiology and Bosch Institute, University of Sydney, 2Motor Neuron Disease Research Group, Australian School of Advanced Medicine, Macquarie University, 3Advanced Microscopy Facility, Bosch Institute, University of Sydney

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The neuromuscular junction (NMJ) is altered in a variety of conditions that can sometimes culminate in synaptic failure. This report describes fluorescence microscope-based methods to quantify such structural changes.

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Neuromuscular Junction Synapse Size Fragmentation Synaptic Protein Density Confocal Fluorescence Microscopy Cholinergic Relay Synapse Motor Neurons Muscle Contraction Structural Changes Neurotransmission Failure Weakness Atrophy Muscle Fiber Genetic Modifications Disease Mouse NMJ Maximum Intensity Projection Confocal Images Acetylcholine Receptor (AChR) Synaptic Vesicle Staining Immunostaining For Synaptic Proteins Fluorescence Resonance Energy Transfer (FRET) Packing Of Postsynaptic AChRs Endplate Protocols Normative Data
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