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Neuromuscular צומת: גודל סינפסה מדידה, שינויי פיצול ובצפיפות חלבון Synaptic שימוש Confocal מיקרוסקופ פלואורסצנטי
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JoVE Journal Neuroscienze
The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy
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Neuromuscular צומת: גודל סינפסה מדידה, שינויי פיצול ובצפיפות חלבון Synaptic שימוש Confocal מיקרוסקופ פלואורסצנטי

21,074 Views

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12:18 min

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December 26, 2014

DOI:

10.3791/52220-v

DOI:
10.3791/52220-v

•

12:18 min
December 26, 2014

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10 Views
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1Physiology and Bosch Institute,University of Sydney, 2Motor Neuron Disease Research Group, Australian School of Advanced Medicine,Macquarie University, 3Advanced Microscopy Facility, Bosch Institute,University of Sydney

Tags

Neuromuscular JunctionSynapseConfocal MicroscopyAcetylcholine ReceptorSynaptic VesicleSynaptic ProteinFRETNeurotransmissionMuscle ContractionStructural ChangesMouse Model

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Tse, N., Morsch, M., Ghazanfari, N., Cole, L., Visvanathan, A., Leamey, C., Phillips, W. D. The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy. J. Vis. Exp. (94), e52220, doi:10.3791/52220 (2014).

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