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In Analisi Vitro di Myd88 mediata Cellular Immune Response to West Nile virus mutante Strain Infezione
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
DOI:

10:39 min

November 27, 2014

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Capitoli

  • 00:05Titolo
  • 01:40Flow Cytometry Analysis In Vitro OTII CD4+ T Cell Priming by DC from WNV-infected Mice
  • 05:00Intracellular Cytokine Staining
  • 07:13Results: DC Function and T Cell Responses at an Early Stage of WNV NS4B–P38G Infection
  • 09:45Conclusion

Summary

Traduzione automatica

Two flow cytometry-based methods – an in vitro T cell priming assay and intracellular cytokine staining were utilized to measure antigen presenting capacity of dendritic cells and antigen-specific T cell responses to a West Nile virus mutant infection in mice.

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