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Brain Slice Biotinylatie: An<em> Ex Vivo</em> Aanpak Regiospecifieke van plasma membraaneiwit Trafficking Meet in Adult Neuronen
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JoVE Journal Neuroscienze
Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons
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Brain Slice Biotinylatie: An<em> Ex Vivo</em> Aanpak Regiospecifieke van plasma membraaneiwit Trafficking Meet in Adult Neuronen

12,752 Views

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06:18 min

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April 03, 2014

DOI:

10.3791/51240-v

DOI:
10.3791/51240-v

•

06:18 min
April 03, 2014

•

5 Views
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1Program in Neuroscience, Graduate School of Biomedical Sciences,University of Massachusetts Medical School, 2Brudnick Neuropsychiatric Research Institute, Department of Psychiatry,University of Massachusetts Medical School

Summary

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Neuronale membraan mensenhandel stelt dynamisch plasmamembraaneiwit beschikbaarheid en significant invloed neurotransmissie. Tot op heden, is het een uitdaging om neuronale endocytische handel in volwassen neuronen te meten. Hier beschrijven we een zeer effectieve, kwantitatieve methode om snelle veranderingen in de oppervlakte-eiwit expressie ex vivo bij acute plakjes hersenen te meten.

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Cite this Article

Gabriel, L. R., Wu, S., Melikian, H. E. Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons. J. Vis. Exp. (86), e51240, doi:10.3791/51240 (2014).

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