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Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons
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JoVE Journal Neurowissenschaften
Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

12,752 Views

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06:18 min

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April 03, 2014

DOI:

10.3791/51240-v

DOI:
10.3791/51240-v

•

06:18 min
April 03, 2014

•

5 Views
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1Program in Neuroscience, Graduate School of Biomedical Sciences,University of Massachusetts Medical School, 2Brudnick Neuropsychiatric Research Institute, Department of Psychiatry,University of Massachusetts Medical School

Summary

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Neuronal membrane trafficking dynamically controls plasma membrane protein availability and significantly impacts neurotransmission. To date, it has been challenging to measure neuronal endocytic trafficking in adult neurons. Here, we describe a highly effective, quantitative method to measure rapid changes in surface protein expression ex vivo in acute brain slices.

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Brain SliceBiotinylationEx VivoPlasma Membrane ProteinTraffickingAdult NeuronsEndocytic TraffickingSurface BiotinylationCultured CellsRegion-specificNeuromodulatory EventsReceptorIon ChannelTransporterCell Surface Presentation

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Cite this Article

Gabriel, L. R., Wu, S., Melikian, H. E. Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons. J. Vis. Exp. (86), e51240, doi:10.3791/51240 (2014).

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