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Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons
Chapters
- 00:05Title
- 01:17Prepare Brain Slices
- 02:25Drug Treatment, Slice Biotinylation, and Tissue Lysate Preparation
- 04:35Results: Biotinylation of Brain Slices
- 05:55Conclusion
Neuronal membrane trafficking dynamically controls plasma membrane protein availability and significantly impacts neurotransmission. To date, it has been challenging to measure neuronal endocytic trafficking in adult neurons. Here, we describe a highly effective, quantitative method to measure rapid changes in surface protein expression ex vivo in acute brain slices.
