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Neuroscience

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JoVE Journal Neuroscience
Brain Slice Biotinylation
 

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

Article DOI: 10.3791/51240-v • 06:18 min • April 3rd, 2014
April 3rd, 2014 •
1, 1, 2
1Program in Neuroscience, Graduate School of Biomedical Sciences, University of Massachusetts Medical School, 2Brudnick Neuropsychiatric Research Institute, Department of Psychiatry, University of Massachusetts Medical School

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Neuronal membrane trafficking dynamically controls plasma membrane protein availability and significantly impacts neurotransmission. To date, it has been challenging to measure neuronal endocytic trafficking in adult neurons. Here, we describe a highly effective, quantitative method to measure rapid changes in surface protein expression ex vivo in acute brain slices.

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Brain Slice Biotinylation Ex Vivo Approach Plasma Membrane Protein Trafficking Adult Neurons Endocytic Trafficking Surface Biotinylation Membrane Protein Surface Expression Exogenous Stimuli Gene Manipulation Cultured Cells Region-specific Differences Acute Brain Slice Preparation Rapid Changes High-fidelity Approach Neuronal Endocytic Trafficking
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