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Metodología Para La Generación Eficiente De Proteínas Del Virus Vaccinia Marcadas Fluorescentemente
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
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Metodología Para La Generación Eficiente De Proteínas Del Virus Vaccinia Marcadas Fluorescentemente

7,281 Views

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09:27 min

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January 17, 2014

DOI:

10.3791/51151-v

DOI:
10.3791/51151-v

•

09:27 min
January 17, 2014

•

2 Views
, , , , ,
1School of Molecular Bioscience,University of Sydney, 2Department of Medicine,Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science,University of Melbourne

Summary

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Un protocolo rápido y modular para la generación de virus recombinantes de la vacuna que expresan las proteínas fluorescente marcadas simultáneamente usando el método de selección dominante transitoria se describe aquí.

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Cite this Article

Marzook, N. B., Procter, D. J., Lynn, H., Yamamoto, Y., Horsington, J., Newsome, T. P. Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins. J. Vis. Exp. (83), e51151, doi:10.3791/51151 (2014).

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