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有效生成荧光标记疫苗病毒蛋白的方法
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
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有效生成荧光标记疫苗病毒蛋白的方法

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09:27 min

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January 17, 2014

DOI:

10.3791/51151-v

DOI:
10.3791/51151-v

•

09:27 min
January 17, 2014

•

2 Views
, , , , ,
1School of Molecular Bioscience,University of Sydney, 2Department of Medicine,Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science,University of Melbourne

Summary

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此处描述了一种快速和模块化的协议，用于生成重组疫苗病毒，同时使用瞬时主导选择的方法表达荧光标记的蛋白质。

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Cite this Article

Marzook, N. B., Procter, D. J., Lynn, H., Yamamoto, Y., Horsington, J., Newsome, T. P. Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins. J. Vis. Exp. (83), e51151, doi:10.3791/51151 (2014).

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