/

Immunologia e infezioni

/

自动分离<em> C。线虫</em>可多段由细菌病原体殖民者

JoVE Journal
Immunologia e infezioni

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunologia e infezioni

Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

Please note that all translations are automatically generated. Click here for the English version.

自动分离<em> C。线虫</em>可多段由细菌病原体殖民者

DOI:

10.3791/51090-v

•

11:18 min

•

March 21, 2014

•

Kwame Twumasi-Boateng, Maureen Berg, Michael Shapira

¹Department of Integrative Biology,University of California, Berkeley

Capitoli

00:05Titolo
01:49Infection of Synchronized Young Adult Worms with Pseudomonas aeruginosa
04:44Setting up the Wormsorter
06:19Sorting Colonized vs Noncolonized Worms
08:28Results: Colonization is Not Required for the Transcriptional Response to P. aeruginosa
10:19Conclusion

Summary

Traduzione automatica

该wormsorter通过根据荧光记者表达分拣蠕虫有利于遗传筛选在秀丽隐杆线虫 。在这里，我们描述了一个新的使用：根据定植通过GFP表达病原体排序，我们用它来检查病原体识别在启动免疫反应的了解甚少的作用。

Tags

C. Elegans Caenorhabditis Elegans Bacterial Pathogen Wormsorter FACS GFP Pseudomonas Aeruginosa Immune Response Pathogen Colonization Pathogen-associated Molecular Patterns Damage Detection

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

含量为病原体相关分子模式（PAMP）触发免疫功能的植物（公共交通交汇处）

由细菌病原体入侵人体细胞

唾液，唾液腺，淋巴收集肩突硬蜱蜱

细菌毒素引起的响应，用活细胞荧光显微镜的可视化

成像InlC的分泌由细菌病原体调查感染蜂窝<em>单核细胞增生李斯特氏菌</em

生成和多表型高内涵筛选<em>贝氏柯克斯体</em>转座子突变体

从非耕种植物病原菌利用酵母双杂交筛选Unravelling一个细菌效应中的作用

质谱法富集细菌脂蛋白及 n-端脂肽的制备结构测定

一种高通量、高含量、液态的 Pathosystem线虫

利用广域跟踪平台对大型线虫种群进行自动行为分析

Read Article