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体外 共生分析评估病原体诱导中性粒细胞跨上皮迁移
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

体外 共生分析评估病原体诱导中性粒细胞跨上皮迁移

DOI:

14:14 min

January 06, 2014

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Capitoli

  • 00:05Titolo
  • 02:01Collagen Coating Transwells
  • 03:25Seeding Collagen Coated Transwells with Epithelial Cells
  • 04:30Preparation of Epithelial Cell Layers for Migration Assay
  • 06:01Neutrophil Trans-epithelial Migration Assay
  • 11:27Results: Assessing Neutrophil Trans-epithelial Migration
  • 13:23Conclusion

Summary

Traduzione automatica

中性粒细胞跨上皮迁移,以应对粘膜细菌感染,有助于上皮损伤和临床疾病。已经开发出一种 体外 模型,结合了病原体、人类嗜中性粒细胞和在转井过滤器上生长的极化人类上皮细胞层,以促进研究,以解开协调这一现象的分子机制。

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