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Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells

DOI:
10.3791/50561-v

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12:43 min

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January 06, 2014

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, , , , , , , , ,
1Department of Surgery,University of Michigan, 2Department of Internal Medicine,University of Michigan, 3Department of Otolaryngology,University of Michigan

Capitoli

  • 00:05Titolo
  • 02:12ALDEFLUOR Staining
  • 04:45Preparation of Cancer Stem Cell Lysate-pulsed Dendritic Cell (CSC-TPDC) Vaccine
  • 07:11Vaccination and Evaluation of the Efficacy
  • 09:07Results: CSC-TPDC Vaccination Significantly Inhibits Murine Tumor Growth Activity
  • 12:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

Evaluated in syngeneic immunocompetent hosts, cancer stem cell (CSC) based dendritic cell (DC) vaccine demonstrated significantly higher antitumor immunity than traditional DC vaccines pulsed with heterogeneous bulk tumor cells.

Tags

Keywords: Cancer Stem CellsDendritic Cell VaccineMelanomaSquamous CancerALDHAntitumor ImmunityIFN-γGM-CSF

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