Journal
/
Immunology and Infection
/
Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells

DOI:
10.3791/50561-v

•

12:43 min

•

January 06, 2014

•

, , , , , , , , ,
1Department of Surgery,University of Michigan, 2Department of Internal Medicine,University of Michigan, 3Department of Otolaryngology,University of Michigan

Chapters

  • 00:05Title
  • 02:12ALDEFLUOR Staining
  • 04:45Preparation of Cancer Stem Cell Lysate-pulsed Dendritic Cell (CSC-TPDC) Vaccine
  • 07:11Vaccination and Evaluation of the Efficacy
  • 09:07Results: CSC-TPDC Vaccination Significantly Inhibits Murine Tumor Growth Activity
  • 12:07Conclusion

Summary

Automatic Translation

Automatic Translation

Evaluated in syngeneic immunocompetent hosts, cancer stem cell (CSC) based dendritic cell (DC) vaccine demonstrated significantly higher antitumor immunity than traditional DC vaccines pulsed with heterogeneous bulk tumor cells.

Tags

Cancer Stem CellsDendritic Cell VaccineMelanomaSquamous CancerALDHAntitumor ImmunityIFN-γGM-CSF

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method

Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

Read Article