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Isolation des neurones sensoriels de<em> Aplysia californica</em> Patch pour les enregistrements de serrage des courants glutamatergiques
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Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
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Isolation des neurones sensoriels de<em> Aplysia californica</em> Patch pour les enregistrements de serrage des courants glutamatergiques

12,132 Views

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09:40 min

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July 10, 2013

DOI:

10.3791/50543-v

DOI:
10.3791/50543-v

•

09:40 min
July 10, 2013

•

8 Views
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1Division of Marine Biology and Fisheries, Rosenstiel School of Marine and Atmospheric Sciences,University of Miami

Summary

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Nous décrivons la dissection du système nerveux du lièvre marin mer<em> Aplysie</em> Après anesthésie, l'isolement de neurones pour la culture des tissus court terme, et des enregistrements de courants ioniques de cellules isolées par la technique de patch-clamp.

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Fieber, L. A., Carlson, S. L., Kempsell, A. T., Greer, J. B., Schmale, M. C. Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents. J. Vis. Exp. (77), e50543, doi:10.3791/50543 (2013).

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