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制备肿瘤抗原负载成熟树突状细胞免疫治疗
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
DOI:

08:40 min

August 01, 2013

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Capitoli

  • 00:05Titolo
  • 01:09Isolation and Cryopreservation of PBMCs
  • 03:09Day 0: Differentiation of Dendritic Cells from Monocytes
  • 04:33Day 5: Harvesting of Immature Dendritic Cells
  • 05:21Day 6: Maturation and Antigen Loading of Dendritic Cells
  • 05:58Day 7: Cryopreservation of Dendritic Cells
  • 07:13Results: Poly-ICLC Treatment Induces PBMC Differentiation into Functionally Mature DCs
  • 08:14Conclusion

Summary

Traduzione automatica

用于产生在肿瘤免疫治疗中使用的大量的自体树突状细胞(DC)的最常用的方法进行说明。该方法使用的IL-4和GM-CSF分化的单核细胞的树突状。未成熟DC刺激才成熟,然后装入抗原注射到病人。

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