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Immunology and Infection

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JoVE Journal Immunology and Infection
Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
 

Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy

Article DOI: 10.3791/50085-v • 08:40 min • August 1st, 2013
August 1st, 2013 •
1, 2, 1, 1, 1, 1
1Cancer Institute, NYU Langone Medical Center, 2Infectious Diseases, NYU Langone Medical Center

Capítulos

Resumen
Automatic Translation

The most commonly used method for generating large numbers of autologous dendritic cells (DCs) for use in tumor immunotherapy is described. The method uses IL-4 and GM-CSF to differentiate DCs from monocytes. The immature DCs are stimulated to mature and then loaded with antigens before they are injected back into the patient.

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Tumor Antigen-loaded Mature Dendritic Cells Immunotherapy Clinical Studies Antigen-loaded DC Vaccines Good Manufacturing Process (GMP) Ex Vivo Preparation Method Synthetic TLR 3 Agonist Polyinosinic-Polycytidylic Acid-poly-L-lysine Carboxymethylcellulose (Poly-ICLC) CD83 CD86 Interleukin-12 (IL-12) Tumor Necrosis Factor (TNF) Interferon Gamma-induced Protein 10 (IP-10) Interleukin 1 (IL-1) Type I Interferons (IFN) Interleukin 10 (IL-10) Peripheral Blood Mononuclear Cells (PBMCs) Leukapheresis Ficoll Gradient Centrifugation Monocytes
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