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JoVE Journal Neuroscience
Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
 

Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices

Article DOI: 10.3791/50034-v • 10:04 min • October 29th, 2012
October 29th, 2012 •
1, 1, 1
1Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University

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In this paper, we describe a useful method to study ligand-gated ion channel function in neurons of acutely isolated brain slices. This method involves the use of a drug-filled micropipette for local application of drugs to neurons recorded using standard patch clamp techniques.

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Local Application Drugs Study Nicotinic Acetylcholine Receptor Function Mouse Brain Slices Tobacco Use Health Problems Cancer Heart Disease Emphysema Stroke Addiction Cigarette Smoking Neuropsychiatric Disorder Biophysical Actions Cellular Actions Nicotine Central Nervous System NAChR Subtypes Brain Areas Nicotine Addiction Electrophysiology Techniques Whole-cell Patch Clamp Recordings Two-electrode Voltage Clamp Recordings Pharmacological Characterization Mammalian Tissue Culture Cells Xenopus Laevis Oocytes Modern Pharmacology Native Environment Brain Slices Laboratory Mice Or Rats
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