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Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
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Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
Chapitres
- 00:05Titre
- 01:46Acute Brain Slice Preparation and Patch Clamp Recording
- 03:47Local Application of Drugs to Neurons in Slices
- 06:07Controlling the Drug-filled Micropipette with a Piezoelectric Translator
- 07:22Results: Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
- 09:33Conclusion
In this paper, we describe a useful method to study ligand-gated ion channel function in neurons of acutely isolated brain slices. This method involves the use of a drug-filled micropipette for local application of drugs to neurons recorded using standard patch clamp techniques.
