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Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
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Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
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October 29, 2012
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In this paper, we describe a useful method to study ligand-gated ion channel function in neurons of acutely isolated brain slices. This method involves the use of a drug-filled micropipette for local application of drugs to neurons recorded using standard patch clamp techniques.
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Engle, S. E., Broderick, H. J., Drenan, R. M. Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices. J. Vis. Exp. (68), e50034, doi:10.3791/50034 (2012).
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