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播散性念珠菌在斑马鱼幼虫的非侵入性成像
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae
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播散性念珠菌在斑马鱼幼虫的非侵入性成像

DOI:
10.3791/4051-v

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10:45 min

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July 30, 2012

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,
1Department of Molecular and Biomedical Sciences,University of Maine

Capitoli

  • 00:05Titolo
  • 01:30Fungal Culture Preparation
  • 03:13Zebrafish Infections
  • 08:25Preparing the Fish for Imaging
  • 09:24Results: Zebrafish Hindbrain Ventricle Infection with Candida albicans
  • 10:12Conclusion

Summary

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斑马鱼的快速发展，小尺寸和透明度是先天感染的免疫控制的研究的巨大优势<sup> 1-4</sup>。在这里，我们证明斑马鱼幼虫病原真菌感染的技术<em>白念珠菌</em>通过显微注射方法最近使用的牵连吞噬细胞NADPH氧化酶活性真菌异形控制<sup> 5</sup>。

Tags

Non-invasive ImagingDisseminated CandidiasisZebrafish LarvaeCandida AlbicansInnate ImmunityGenetic DefectsPhagocyte NADPH OxidaseDynamics Of C. Albicans InteractionMacrophagesNeutrophilsIn Vitro StudiesIn Vivo EnvironmentCytokine LevelsExtracellular Matrix AttachmentsIntercellular ContactsModel OrganismLive Intact HostZebrafish LarvaInnate Immune Defenses

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