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复用荧光免疫检测方法和故障排除
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

复用荧光免疫检测方法和故障排除

DOI:

08:05 min

December 12, 2011

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Capitoli

  • 00:05Titolo
  • 01:38The MFIA Reagent and Sample Preparation
  • 02:47Test Plate and MFIA Bead Suspension Preparation
  • 03:49Addition of Sera, Washing and Reading the Plate
  • 06:05Representative MFIA Testing Results
  • 07:07Conclusion

Summary

Traduzione automatica

使用Luminex公司的xMAP微球技术,我们开发了复用荧光免疫法(MFIA)为血清学监测各种实验室动物物种的。 MFIA是抗原,组织控制或免疫球蛋白共价连接到颜色编码的聚苯乙烯微球的悬浮液微阵列。解决MFIA测试方法以及各种疑难排解主题。

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