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Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
Kapitel
- 00:05Titel
- 01:38The MFIA Reagent and Sample Preparation
- 02:47Test Plate and MFIA Bead Suspension Preparation
- 03:49Addition of Sera, Washing and Reading the Plate
- 06:05Representative MFIA Testing Results
- 07:07Conclusion
Using Luminex Corporation’s xMAP microsphere technology, we have developed the Multiplexed Fluorometric ImmunoAssay (MFIA) for serosurveillance of various laboratory animal species. The MFIA is a suspension microarray where antigen, tissue control or immunoglobulins are covalently linked to color-coded polystyrene microspheres. The MFIA testing method as well as various troubleshooting topics is addressed.
Tags
Multiplexed Fluorometric ImmunoAssayDiagnostic TestingAnimal ModelsBiomedical ResearchInfectious AgentsVirusesMycoplasmaMicroorganismsSerologyAntibody ResponsesSerosurveillanceEnzyme-linked Immunosorbent Assay (ELISA)Multiplexed AssayMicrospheresColor SetsSerumReagentsDisposablesInternal Control BeadsIgG Anti-test Serum Species Immunoglobulin (αIg)
