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Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
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December 12, 2011
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Using Luminex Corporation’s xMAP microsphere technology, we have developed the Multiplexed Fluorometric ImmunoAssay (MFIA) for serosurveillance of various laboratory animal species. The MFIA is a suspension microarray where antigen, tissue control or immunoglobulins are covalently linked to color-coded polystyrene microspheres. The MFIA testing method as well as various troubleshooting topics is addressed.
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Multiplexed Fluorometric ImmunoAssayDiagnostic TestingAnimal ModelsBiomedical ResearchInfectious AgentsVirusesMycoplasmaMicroorganismsSerologyAntibody ResponsesSerosurveillanceEnzyme-linked Immunosorbent Assay (ELISA)Multiplexed AssayMicrospheresColor SetsSerumReagentsDisposablesInternal Control BeadsIgG Anti-test Serum Species Immunoglobulin (αIg)
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Wunderlich, M. L., Dodge, M. E., Dhawan, R. K., Shek, W. R. Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting. J. Vis. Exp. (58), e3715, doi:10.3791/3715 (2011).
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