This content is Free Access.
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
28,041 Views
•
08:05 min
•
December 12, 2011
DOI:
Özet
Automatically generated
Using Luminex Corporation’s xMAP microsphere technology, we have developed the Multiplexed Fluorometric ImmunoAssay (MFIA) for serosurveillance of various laboratory animal species. The MFIA is a suspension microarray where antigen, tissue control or immunoglobulins are covalently linked to color-coded polystyrene microspheres. The MFIA testing method as well as various troubleshooting topics is addressed.
Explore More Videos
Multiplexed Fluorometric ImmunoAssayDiagnostic TestingAnimal ModelsBiomedical ResearchInfectious AgentsVirusesMycoplasmaMicroorganismsSerologyAntibody ResponsesSerosurveillanceEnzyme-linked Immunosorbent Assay (ELISA)Multiplexed AssayMicrospheresColor SetsSerumReagentsDisposablesInternal Control BeadsIgG Anti-test Serum Species Immunoglobulin (αIg)
Read Article
Cite this Article
Wunderlich, M. L., Dodge, M. E., Dhawan, R. K., Shek, W. R. Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting. J. Vis. Exp. (58), e3715, doi:10.3791/3715 (2011).
Copy