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Derivation of Glial Restricted Precursors from E13 mice
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June 20, 2012
DOI:
1Hugo W. Moser Research Institute at Kennedy Krieger,Johns Hopkins University, 2Department of Neurology,Johns Hopkins School of Medicine, 3University of Maryland , 4Experimental Neurology,Biogen Idec, 5The Brain Science Institute,Johns Hopkins School of Medicine, 6Department of Pediatrics,Johns Hopkins School of Medicine
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This protocol outlines the derivation of Glial Restricted Precursors from fetal spinal cords and maintained in vitro either for transplantation or for the study of oligodendrocytic lineage.
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Phillips, A. W., Falahati, S., DeSilva, R., Shats, I., Marx, J., Arauz, E., Kerr, D. A., Rothstein, J. D., Johnston, M. V., Fatemi, A. Derivation of Glial Restricted Precursors from E13 mice. J. Vis. Exp. (64), e3462, doi:10.3791/3462 (2012).
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