Journal
/
Neurociencias
/
Derivation of Glial Restricted Precursors from E13 mice
JoVE Revista
Neurociencias
This content is Free Access.
JoVE Revista Neurociencias
Derivation of Glial Restricted Precursors from E13 mice

Derivation of Glial Restricted Precursors from E13 mice

DOI:
10.3791/3462-v

•

08:56 min

•

June 20, 2012

•

, , , , , , , , ,
1Hugo W. Moser Research Institute at Kennedy Krieger,Johns Hopkins University, 2Department of Neurology,Johns Hopkins School of Medicine, 3University of Maryland , 4Experimental Neurology,Biogen Idec, 5The Brain Science Institute,Johns Hopkins School of Medicine, 6Department of Pediatrics,Johns Hopkins School of Medicine

Capítulos

  • 00:05Título
  • 01:05Tissue Derivation
  • 03:48Cell Dissociation and Culture Establishment
  • 05:19Immunopanning the GRP Population
  • 06:27Freezing and Storage of Derived Cells
  • 07:24Purified GRP Cells
  • 08:27Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol outlines the derivation of Glial Restricted Precursors from fetal spinal cords and maintained in vitro either for transplantation or for the study of oligodendrocytic lineage.

Tags

Glial Restricted PrecursorGRP CellsWhite Matter DiseasesPeriventricular LeukomalaciaPVLOligodendrocyte Progenitor CellsOPCImmature OligodendrocytesPreOLHypoxia-ischemiaOxidative StressExcitotoxicityGlutamate ExcitotoxicityOligodendrocyte ProgenitorsTransplantation Experiments

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues

Isolation and Culture of Hippocampal Neurons from Prenatal Mice

Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue

Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Cannula Implantation into the Cisterna Magna of Rodents

Recording Spatially Restricted Oscillations in the Hippocampus of Behaving Mice

Read Article