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Derivation of Glial Restricted Precursors from E13 mice
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Derivation of Glial Restricted Precursors from E13 mice

Derivation of Glial Restricted Precursors from E13 mice

DOI:
10.3791/3462-v

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08:56 min

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June 20, 2012

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, , , , , , , , ,
1Hugo W. Moser Research Institute at Kennedy Krieger,Johns Hopkins University, 2Department of Neurology,Johns Hopkins School of Medicine, 3University of Maryland , 4Experimental Neurology,Biogen Idec, 5The Brain Science Institute,Johns Hopkins School of Medicine, 6Department of Pediatrics,Johns Hopkins School of Medicine

Chapters

  • 00:05Title
  • 01:05Tissue Derivation
  • 03:48Cell Dissociation and Culture Establishment
  • 05:19Immunopanning the GRP Population
  • 06:27Freezing and Storage of Derived Cells
  • 07:24Purified GRP Cells
  • 08:27Conclusion

Summary

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This protocol outlines the derivation of Glial Restricted Precursors from fetal spinal cords and maintained in vitro either for transplantation or for the study of oligodendrocytic lineage.

Tags

Glial Restricted PrecursorGRP CellsWhite Matter DiseasesPeriventricular LeukomalaciaPVLOligodendrocyte Progenitor CellsOPCImmature OligodendrocytesPreOLHypoxia-ischemiaOxidative StressExcitotoxicityGlutamate ExcitotoxicityOligodendrocyte ProgenitorsTransplantation Experiments

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