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Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
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JoVE Journal Neuroscienze
Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells

Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells

DOI:
10.3791/2380-v

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10:12 min

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November 27, 2010

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, , , , , ,
1Department of Pediatric Oncology,University Children’s Hospital Essen, 2Department of Developmental Pathology,Bonn Medical School, Institute of Pathology

Capitoli

  • 00:01Titolo
  • 00:19Introduction
  • 00:41Dissection of Chick Embryos
  • 02:54Maceration of Chick Embryos
  • 03:59Centrifugation of Chick Embryos
  • 08:30Conclusions

Summary

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To cultivate neural crest stem cells (NCSC) in vitro, a special medium (NCSCM) is required. Essential part of NCSCM is chick embryo extract (CEE). We here describe accurate techniques to produce a maximized amount of pure and high quality CEE, including details as the isolation, maceration, centrifugation, and filtration processes.

Tags

Chick Embryo ExtractMurine Neural Crest Stem CellsNeuro-ectodermPluripotent Progenitor CellsP75SOX10MigrationSmooth Muscle CellsChromaffin CellsNeuronsGlial CellsMelanocytesCartilageBoneNeural Crest Stem Cell Medium (NCSCM)Growth FactorsChick Embryo Extract PreparationIsolationMacerationCentrifugationFiltration Processes

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