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Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
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Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
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10:12 min
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November 27, 2010
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Summary
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To cultivate neural crest stem cells (NCSC) in vitro, a special medium (NCSCM) is required. Essential part of NCSCM is chick embryo extract (CEE). We here describe accurate techniques to produce a maximized amount of pure and high quality CEE, including details as the isolation, maceration, centrifugation, and filtration processes.
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Chick Embryo ExtractMurine Neural Crest Stem CellsNeuro-ectodermPluripotent Progenitor CellsP75SOX10MigrationSmooth Muscle CellsChromaffin CellsNeuronsGlial CellsMelanocytesCartilageBoneNeural Crest Stem Cell Medium (NCSCM)Growth FactorsChick Embryo Extract PreparationIsolationMacerationCentrifugationFiltration Processes
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Pajtler, K., Bohrer, A., Maurer, J., Schorle, H., Schramm, A., Eggert, A., Schulte, J. H. Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells. J. Vis. Exp. (45), e2380, doi:10.3791/2380 (2010).
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