/
/
Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
12,566 Views
•
10:12 min
•
November 27, 2010
DOI:
Summary
Automatically generated
To cultivate neural crest stem cells (NCSC) in vitro, a special medium (NCSCM) is required. Essential part of NCSCM is chick embryo extract (CEE). We here describe accurate techniques to produce a maximized amount of pure and high quality CEE, including details as the isolation, maceration, centrifugation, and filtration processes.
Explore More Videos
Chick Embryo ExtractMurine Neural Crest Stem CellsNeuro-ectodermPluripotent Progenitor CellsP75SOX10MigrationSmooth Muscle CellsChromaffin CellsNeuronsGlial CellsMelanocytesCartilageBoneNeural Crest Stem Cell Medium (NCSCM)Growth FactorsChick Embryo Extract PreparationIsolationMacerationCentrifugationFiltration Processes
Read Article
Cite this Article
Pajtler, K., Bohrer, A., Maurer, J., Schorle, H., Schramm, A., Eggert, A., Schulte, J. H. Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells. J. Vis. Exp. (45), e2380, doi:10.3791/2380 (2010).
Copy