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Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
Chapters
- 00:01Title
- 00:19Introduction
- 00:41Dissection of Chick Embryos
- 02:54Maceration of Chick Embryos
- 03:59Centrifugation of Chick Embryos
- 08:30Conclusions
To cultivate neural crest stem cells (NCSC) in vitro, a special medium (NCSCM) is required. Essential part of NCSCM is chick embryo extract (CEE). We here describe accurate techniques to produce a maximized amount of pure and high quality CEE, including details as the isolation, maceration, centrifugation, and filtration processes.
Tags
Chick Embryo ExtractMurine Neural Crest Stem CellsNeuro-ectodermPluripotent Progenitor CellsP75SOX10MigrationSmooth Muscle CellsChromaffin CellsNeuronsGlial CellsMelanocytesCartilageBoneNeural Crest Stem Cell Medium (NCSCM)Growth FactorsChick Embryo Extract PreparationIsolationMacerationCentrifugationFiltration Processes
