Rivista
/
Immunologia e infezioni
/
Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei
/
Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei

Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei

34 Views

•

00:54 min

•

January 19, 2024

DOI:

10.3791/201368-v

DOI:
10.3791/201368-v

•

00:54 min
January 19, 2024

•

2 Views
, , , , , ,
1Department Chemistry and Biochemistry,Brigham Young University, 2Eukaryotic Pathogens Innovation Center,Clemson University, 3Department of Genetics and Biochemistry,Clemson University, 4Department of Physics and Astronomy,Clemson University

Explore More Videos

Flow CytometryPHluorin2Trypanosoma BruceiSample PreparationTetracyclineDoxycyclineCell CultureCentrifugationPBSGlucosePropidium IodideThiazole RedLive/dead Staining

Read Article

Cite this Article

Call, D., Pizarro, S. S., Tovey, E., Knight, E., Baumgardner, C., Christensen, K. A., Morris, J. C. Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei. J. Vis. Exp. (Pending Publication), e201368, doi:10.3791/201368 (2024).

Download .ris file

Copy

Share Video

.

Copy