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Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei

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Inmunología y contagio

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JoVE Revista Inmunología y contagio

Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei

Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei

27 Views

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00:54 min

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January 19, 2024

DOI:

10.3791/201368-v

DOI:

10.3791/201368-v

•

00:54 min

January 19, 2024

•

1 Views

Daniel Call*¹, Sabrina S. Pizarro*^2,3, Erica Tovey, Emily Knight³, Carrie Baumgardner⁴, Kenneth A. Christensen, James C. Morris

¹Department Chemistry and Biochemistry,Brigham Young University, ²Eukaryotic Pathogens Innovation Center,Clemson University, ³Department of Genetics and Biochemistry,Clemson University, ⁴Department of Physics and Astronomy,Clemson University

Tags

Flow Cytometry PHluorin2 Trypanosoma Brucei Sample Preparation Tetracycline Doxycycline Cell Culture Centrifugation PBS Glucose Propidium Iodide Thiazole Red Live/dead Staining

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